Rabbit anti-Human Vimentin Monoclonal Antibody | anti-VIM antibody

Recombinant Anti-Vimentin Antibody, Rabbit Monoclonal

Cat. Num. AAA254162

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A
• Synonyms: Vimentin, Antibody; Recombinant Anti-Vimentin Antibody, Rabbit Monoclonal; Vimentin/VIM Antibody, Rabbit MAb; vimentin; Anti-CTRCT30 Antibody; Anti-HEL113 Antibody; anti-VIM antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Human Vimentin/VIM
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VIM antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: A synthetic peptide corresponding to the center region of the Human Vimentin/VIM
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Vimentin/VIM.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunochemical staining of human Vimentin in human lung cancer with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemisry)

(Immunochemical staining of human Vimentin in human malignant melanoma with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohiostchemistry)

(Immunochemical staining of human Vimentin in human stomach with rabbit monoclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-VIM rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: A549 Whole Cell LysateLane C: 293T Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:55 kDa)

Related Product Information for anti-VIM antibody

Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central alpha-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.

References

Russell RL, et al. (2001) Uridine phosphorylase association with vimentin. Intracellular distribution and localization. J Biol Chem. 276(16):13302-7.Moinova, et al. (2012) Aberrant Vimentin Methylation is Characteristic of Upper GI Pathologies. Cancer Epidemiology Biomarkers Prev. 21(4):594-600.Leader M, et al. (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology. 11(1):63-72.

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NM_003380.3
• UniProt Accession #: P08670; Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
• Molecular Weight: 53,652 Da
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN