Mouse Vimentin Monoclonal Antibody | anti-VIM antibody

Vimentin (Mesenchymal Cell Marker)

Cat. Num. AAA215255

• Reactivity: Human; Does not react with Mouse or Rat.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Vimentin, Antibody; Vimentin (Mesenchymal Cell Marker); VIM; anti-VIM antibody

• Host: Mouse
• Reactivity: Human; Does not react with Mouse or Rat.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: VIM/3736
• Specificity: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 466

• Applicable Applications for anti-VIM antibody: IHC (Immunohistochemistry)
• Positive Control: U87, A375, Raji, HEP2 cells. Sarcomas or Melanomas.
• Immunogen: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 10p12.33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 21,000 full-length human proteins using Vimentin Mouse Monoclonal Antibody (VIM/3736) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736).)

References

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

NCBI and Uniprot Product Information

• NCBI GI #: 62414289
• NCBI GeneID: 7431
• NCBI Accession #: NP_003371.2
• NCBI GenBank Nucleotide #: NP_003371.2
• UniProt Accession #: P08670
• Molecular Weight: 57-60kDa
• NCBI Official Full Name: vimentin
• NCBI Official Synonym Full Names: vimentin
• NCBI Official Symbol: VIM
• NCBI Protein Information: vimentin
• UniProt Protein Name: Vimentin
• UniProt Gene Name: VIM
• UniProt Entry Name: VIME_HUMAN