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WB (Western Blot)
(Western Blot; Sample: Lane1: Canine Heart lysate; Lane2: Gallus Heart lysate; Lane3: Porcine Heart lysate; Lane4: Bovine Heart lysate; Lane5: Caprine Heart lysate; Lane6: Caprine Heart lysatePrimary Ab: 0.1ug/ml Mouse Anti-human VIM AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse Vimentin (VIM) Monoclonal Antibody | anti-VIM antibody
Monoclonal Antibody to Vimentin (VIM)
Reactivity
Human, Rabbit, Dog, Pig, Bovine, Goat, Sheep, Chicken
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Chromatography
Synonyms
Vimentin (VIM), Antibody; Monoclonal Antibody to Vimentin (VIM); Vimentin; anti-VIM antibody
Host
Mouse
Reactivity
Human, Rabbit, Dog, Pig, Bovine, Goat, Sheep, Chicken
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C5
Specificity
The antibody is a mouse monoclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Ser2~Glu466
Applicable Applications for anti-VIM antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Immunogen
Recombinant Vimentin (VIM)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: Canine Heart lysate; Lane2: Gallus Heart lysate; Lane3: Porcine Heart lysate; Lane4: Bovine Heart lysate; Lane5: Caprine Heart lysate; Lane6: Caprine Heart lysatePrimary Ab: 0.1ug/ml Mouse Anti-human VIM AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Canine Heart lysate; Lane2: Gallus Heart lysate; Lane3: Porcine Heart lysate; Lane4: Bovine Heart lysate; Lane5: Caprine Heart lysate; Lane6: Caprine Heart lysatePrimary Ab: 0.1ug/ml Mouse Anti-human VIM AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IF (Immunofluorescence)
(FITC staining on IF;Sample: human MCF7 cell; Primary Ab: 30ug/ml Mouse Anti-human VIM AntibodySecond Ab: 1ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IF (Immunofluorescence)
(FITC staining on IF;Sample: human MCF7 cell; Primary Ab: 30ug/ml Mouse Anti-human VIM AntibodySecond Ab: 1ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Porcine Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Sample: Porcine Small intestine Tissue; Primary Ab: 20ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Porcine Spleen Tissue; Primary Ab: 20ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Porcine Spleen Tissue; Primary Ab: 20ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Porcine Kidney Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: Porcine Kidney Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Liver cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Liver cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Kidney Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Sample: human Kidney Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: human Breast cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Sample: human Breast cancer Tissue; Primary Ab: 10ug/ml Mouse Anti-human VIM Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-VIM antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,652 Da
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Protein Information
vimentin
UniProt Protein Name
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
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Product Notes
The VIM vim (Catalog #AAA21218) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser2~Glu466. The Monoclonal Antibody to Vimentin (VIM) reacts with Human, Rabbit, Dog, Pig, Bovine, Goat, Sheep, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Vimentin (VIM) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vimentin (VIM), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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