IF (Immunofluorescence)
(Mouse anti Human vinculin antibody, clone V284 used to illustrate vinculin expression on murine retinal cell line 661W by immunofluorescence.Image caption:Morphology of 661 W on the PET membrane for control and after 120 min of applied EF. Cells were labeled for (a) nucleus,(b) vinculin, (c) filamentous actin cytoskeleton, and (d) merged image. The direction of the arrow shows the direction of the cathode and anode positions. Scale bar 50mum.)
Mouse Vinculin Monoclonal Antibody | anti-VCL antibody
Mouse Anti Human Vinculin
Gene Names
VCL; MV; MVCL; CMD1W; CMH15; HEL114
Reactivity
Reacts with: Rat, Rabbit, Chicken, MouseN.B. Antibody reactivity and working conditions may vary between species.
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Synonyms
Vinculin, Antibody; Mouse Anti Human Vinculin; Vinculin antibody (V284); anti-VCL antibody
Host
Mouse
Reactivity
Reacts with: Rat, Rabbit, Chicken, Mouse
N.B. Antibody reactivity and working conditions may vary between species.
N.B. Antibody reactivity and working conditions may vary between species.
Clonality
Monoclonal
Isotype
IgG1
Clone Number
V284
Specificity
VINCULIN
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1066
Applicable Applications for anti-VCL antibody
WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Purified vinculin from human platelets
Carrier Free
Yes
Target Species
Human
Fusion Partners
Spleen cells of immunised mice were fused with cells from the SP2/0 mouse myeloma line.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Histology Positive Control Tissue
Human appendix
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
IF (Immunofluorescence)
(Mouse anti Human vinculin antibody, clone V284 used to illustrate vinculin expression on murine retinal cell line 661W by immunofluorescence.Image caption:Morphology of 661 W on the PET membrane for control and after 120 min of applied EF. Cells were labeled for (a) nucleus,(b) vinculin, (c) filamentous actin cytoskeleton, and (d) merged image. The direction of the arrow shows the direction of the cathode and anode positions. Scale bar 50mum.)
IF (Immunofluorescence)
(Mouse anti Human vinculin antibody, clone V284 used to illustrate vinculin expression on murine retinal cell line 661W by immunofluorescence.Image caption:Morphology of 661 W on the PET membrane for control and after 120 min of applied EF. Cells were labeled for (a) nucleus,(b) vinculin, (c) filamentous actin cytoskeleton, and (d) merged image. The direction of the arrow shows the direction of the cathode and anode positions. Scale bar 50mum.)
WB (Western Blot)
(HeLa whole cell lysate stained with Mouse anti vinculin detected with Goat anti Mouse IgG (H/L):HRP)
WB (Western Blot)
(HeLa whole cell lysate stained with Mouse anti vinculin detected with Goat anti Mouse IgG (H/L):HRP)
IHC (Immunohistochemistry)
(Staining of paraffin embedded formalin fixed Human appendix with Mouse anti Human vinculin)
IHC (Immunohistochemistry)
(Staining of paraffin embedded formalin fixed Human appendix with Mouse anti Human vinculin)
Related Product Information for anti-VCL antibody
Mouse anti Human vinculin antibody, clone V284 recognizes human vinculin, also known as metavinculin. Vinculin is a highly conserved, cytoskeletal protein associated with cell-cell and cell-matrix adhesion. Vinculin is an actin filament (F-actin)-binding protein involved in anchoring F-actin to the membrane.
Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.
Mouse anti Human vinculin antibody, clone V284 detects vinculin as a ~130 kDa band in homogenates of platelets and other cells. It reacts with leucocytes, muscle cells, epithelial cells and fibroblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vinculin isoform VCL
NCBI Official Synonym Full Names
vinculin
NCBI Official Symbol
VCL
NCBI Official Synonym Symbols
MV; MVCL; CMD1W; CMH15; HEL114
NCBI Protein Information
vinculin
UniProt Protein Name
Vinculin
UniProt Gene Name
VCL
UniProt Synonym Gene Names
MV
UniProt Entry Name
VINC_HUMAN
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Product Notes
The VCL vcl (Catalog #AAA50350) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human Vinculin reacts with Reacts with: Rat, Rabbit, Chicken, Mouse N.B. Antibody reactivity and working conditions may vary between species. and may cross-react with other species as described in the data sheet. AAA Biotech's Vinculin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IP (Immunoprecipitation), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Vinculin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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