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ELISA
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Mouse anti-Human VISTA Monoclonal Antibody | anti-VISTA antibody

VISTA Antibody [4C4]

Gene Names
VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A purified
Synonyms
VISTA, Antibody; VISTA Antibody [4C4]; VISTA molecule; VSIR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; VISTA; C10orf54; chromosome 10 open reading frame 54; PD-1H; V-set immunoregulatory receptor; V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation; Chromosome 10 Open Reading Frame 54; anti-VISTA antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4C4
Purity/Purification
Protein A purified
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
311
Applicable Applications for anti-VISTA antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB at 0.25-1 ug/mL
IHC starting at 2 ug/mL
ICC starting at 1 ug/mL
IF start at 2 ug/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Immunogen
VISTA antibody was raised against the extracellular domain of human VISTA.
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.

ELISA

(Titration curve analysis of VISTA antibody to detect recombinant VISTA in ELISA at decreasing concentrations.)

FCM (Flow Cytometry)

(Flow cytometry analysis of VISTA overexpressing HEK293 cells using VISTA antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: VISTA overexpressing HEK293 cells.)

IHC (Immunohistchemistry)

(Immunohistochemistry of VISTA in human lymphoma tissue with VISTA antibody at 2 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of VISTA in human spleen tissue with VISTA antibody at 10 μg/mL.Red: VISTA Antibody [4C4]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of VISTA in human lymphoma tissue with VISTA antibody at 5 μg/mL.Red: VISTA Antibody [4C4]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of VISTA in transfected HEK293 cells with VISTA antibody at 2 μg/mL.Green: VISTA Antibody [4C4]Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of VISTA in transfected HEK293 cells with VISTA antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

WB (Western Blot)

(Western blot analysis of VISTA in overexpressing HEK293 cells with VISTA antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml)

Related Product Information for anti-VISTA antibody
VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
Product Categories/Family for anti-VISTA antibody
References
Mayya V., et al. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46. Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 31 kDa
Observed: 47 kDa
NCBI Official Full Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
VSIR
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
NCBI Protein Information
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
VSIR
UniProt Synonym Gene Names
Sisp-1

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Product Notes

The VISTA vsir (Catalog #AAA11012) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VISTA Antibody [4C4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's VISTA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB at 0.25-1 ug/mL IHC starting at 2 ug/mL ICC starting at 1 ug/mL IF start at 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the VISTA vsir for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VISTA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.