Mouse anti-Human VISTA/GI24 Monoclonal Antibody | anti-VISTA/GI24 antibody

VISTA/GI24 (Negative Regulator of Immune Response)

Cat. Num. AAA215193

• Gene Names: VSIR; B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
• Reactivity: Human
• Applications: ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: VISTA/GI24, Antibody; VISTA/GI24 (Negative Regulator of Immune Response); B7H5; DD1alpha; GI24; PD-1H; PDCD1 homolog; Platelet receptor Gi24; PP2135;Stress-induced secreted protein 1 (SISP1); V domain Ig suppressor of T cell activation;V Set Immuno-regulatory Receptor; anti-VISTA/GI24 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: VISTA/2864
• Specificity: VISTA/GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC s, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kDa extracellular fragment plus a 25-30kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment). It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 311

• Applicable Applications for anti-VISTA/GI24 antibody: ELISA
• Positive Control: Human spleen or placenta tissue (IHC).
• Immunogen: Recombinant full-length human VISTA protein
• Cellular Localization: Cell Surface and Cytoplasmic
• Hu-chromosome Location: 10q22.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to VISTA (VISTA/2864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)

SDS-PAGE

(SDS-PAGE Analysis Purified Monospecific Mouse Monoclonal Antibody to VISTA (VISTA/2864). Confirmation of Integrity and Purity of Antibody.)

References

Lines, J.L., et al. Cancer Immunol Res. 2014;2(6):510-517.

NCBI and Uniprot Product Information

• NCBI GI #: 62339432
• NCBI GeneID: 64115
• NCBI Accession #: NP_071436.1
• NCBI GenBank Nucleotide #: NP_071436.1
• UniProt Accession #: Q9H7M9
• Molecular Weight: 34kDa
• NCBI Official Full Name: V-type immunoglobulin domain-containing suppressor of T-cell activation
• NCBI Official Synonym Full Names: V-set immunoregulatory receptor
• NCBI Official Symbol: VSIR
• NCBI Official Synonym Symbols: B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
• NCBI Protein Information: V-type immunoglobulin domain-containing suppressor of T-cell activation
• UniProt Protein Name: V-type immunoglobulin domain-containing suppressor of T-cell activation
• UniProt Gene Name: C10orf54
• UniProt Synonym Gene Names: Sisp-1
• UniProt Entry Name: VISTA_HUMAN