WB (Western Blot)
(All lanes : Anti-WNT4 Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse lung lysateLane 4: SK-BR-3 whole cell lysateLane 5: MDA-MB-231 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Mouse anti-Human, Mouse WNT4 Monoclonal Antibody | anti-WNT4 antibody
WNT4 Antibody
Gene Names
WNT4; WNT-4; SERKAL
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Synonyms
WNT4, Antibody; WNT4 Antibody; Protein Wnt-4; WNT4; anti-WNT4 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1698CT540.169.185
Specificity
This WNT4 antibody is generated from a mouse immunized with a recombinant protein between 30-351 amino acids from human WNT4.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
351
Applicable Applications for anti-WNT4 antibody
ELISA, WB (Western Blot)
Cellular Location
Secreted, extracellular space, extracellular matrix.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-WNT4 Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse lung lysateLane 4: SK-BR-3 whole cell lysateLane 5: MDA-MB-231 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-WNT4 Antibody at 1:4000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: mouse lung lysateLane 4: SK-BR-3 whole cell lysateLane 5: MDA-MB-231 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-WNT4 antibody
Background: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity). Overexpression may be associated with abnormal proliferation in human breast tissue.
Function: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:15317892, PubMed:16959810, PubMed:18179883, PubMed:18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:15317892, PubMed:16959810, PubMed:18182450). Requiredfor normal lung development and for normal patterning of trachael cartilage rings (By similarity).
Function: Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:15317892, PubMed:16959810, PubMed:18179883, PubMed:18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:15317892, PubMed:16959810, PubMed:18182450). Requiredfor normal lung development and for normal patterning of trachael cartilage rings (By similarity).
Product Categories/Family for anti-WNT4 antibody
References
Testa T.T.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases. Jordan B.K.,et al.Am. J. Hum. Genet. 68:1102-1109 (2001). Clark H.F.,et al.Genome Res. 13:2265-2270 (2003). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-4
NCBI Official Synonym Full Names
Wnt family member 4
NCBI Official Symbol
WNT4
NCBI Official Synonym Symbols
WNT-4; SERKAL
NCBI Protein Information
protein Wnt-4
UniProt Protein Name
Protein Wnt-4
UniProt Gene Name
WNT4
UniProt Entry Name
WNT4_HUMAN
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Product Notes
The WNT4 wnt4 (Catalog #AAA290653) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The WNT4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT4 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT4 wnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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