Mouse anti-Human Y14/RBM8A Monoclonal Antibody | anti-RBM8A antibody

Y14/RBM8A Mouse Monoclonal Antibody [Clone PCRP-RBM8A-1B4]

Cat. Num. AAA216081

• Gene Names: RBM8A; TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: Y14/RBM8A, Antibody; Y14/RBM8A Mouse Monoclonal Antibody [Clone PCRP-RBM8A-1B4]; Binder of OVCA1-1; BOV1A; BOV1B; BOV1C; HSPC114; MDS014; ZRNP1; Ribonucleoprotein RBM8A; RNA-binding protein 8A; RNA-binding protein Y14; anti-RBM8A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG1
• Clone Number: PCRP-RBM8A-1B4
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-RBM8A antibody: WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human RBM8A protein
• Cellular Localization: Nucleus, Nucleus speckle, Cytoplasm, Nucleocytoplasmic shuttling protein
• Positive Control: HeLa or HepG2 cells.
• Hu-chromosome Location: 1q21.1
• Unigene: 728824
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. Y14/RBM8A Mouse Monoclonal Antibody (PCRP-RBM8A-1B4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-RBM8A antibody

Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.

NCBI and Uniprot Product Information

• NCBI GI #: 4826972
• NCBI GeneID: 9939
• NCBI Accession #: NP_005096.1
• NCBI GenBank Nucleotide #: NM_005105.4
• UniProt Accession #: Q9Y5S9; Q6FHD1; Q6IQ40; Q9GZX8; Q9NZI4; B3KQI9
• Molecular Weight: 19,760 Da
• NCBI Official Full Name: RNA-binding protein 8A
• NCBI Official Synonym Full Names: RNA binding motif protein 8A
• NCBI Official Symbol: RBM8A
• NCBI Official Synonym Symbols: TAR; Y14; RBM8; ZNRP; RBM8B; ZRNP1; BOV-1A; BOV-1B; BOV-1C; MDS014; DEL1q21.1; C1DELq21.1
• NCBI Protein Information: RNA-binding protein 8A; BOV-1; RNA binding motif protein 8B; RNA-binding motif protein 8A; RNA-binding protein Y14; binder of OVCA1-1; ribonucleoprotein RBM8; ribonucleoprotein RBM8A
• UniProt Protein Name: RNA-binding protein 8A
• UniProt Gene Name: RBM8A
• UniProt Synonym Gene Names: RBM8; BOV-1
• UniProt Entry Name: RBM8A_HUMAN