WB (Western Blot)
(Western blot analysis of lysates from HeLa cells using [KO Validated] Ythdc1 Rabbit mAb (AAA283064) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
Rabbit anti-Mouse Ythdc1 Monoclonal Antibody | anti-Ythdc1 antibody
[KO Validated] Ythdc1 Rabbit mAb
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
Ythdc1, Antibody; [KO Validated] Ythdc1 Rabbit mAb; mKIAA1966; A730098D12Rik; Mouse Ythdc1; anti-Ythdc1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
Applicable Applications for anti-Ythdc1 antibody
ELISA, WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse Ythdc1 (NP_808348.2).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells using [KO Validated] Ythdc1 Rabbit mAb (AAA283064) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from HeLa cells using [KO Validated] Ythdc1 Rabbit mAb (AAA283064) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb (AAA283064) at 1:1000 dilution incubated overnight at 4?. Ythdc1-cKO mouse ES cells were treated by 4OHT.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
WB (Western Blot)
(Western blot analysis of lysates from Ythdc1-cKO mouse ES cells, using [KO Validated] Ythdc1 Rabbit mAb (AAA283064) at 1:1000 dilution incubated overnight at 4?. Ythdc1-cKO mouse ES cells were treated by 4OHT.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)
Related Product Information for anti-Ythdc1 antibody
Predicted to enable N6-methyladenosine-containing RNA binding activity and mRNA binding activity. Involved in posttranscriptional regulation of gene expression. Acts upstream of or within gamete generation; in utero embryonic development; and regulation of mRNA processing. Located in nucleus. Orthologous to human YTHDC1 (YTH domain containing 1).
Product Categories/Family for anti-Ythdc1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 85kDa
Observed MW: 100kDa
Observed MW: 100kDa
UniProt Protein Name
YTH domain-containing protein 1
UniProt Gene Name
Ythdc1
UniProt Synonym Gene Names
Kiaa1966
UniProt Entry Name
YTDC1_MOUSE
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Product Notes
The Ythdc1 ythdc1 (Catalog #AAA283064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] Ythdc1 Rabbit mAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ythdc1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Ythdc1 ythdc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SYNDYVREFH NMGPPPPWQG MPPYPGIEQP PHHPYYQHHA PPPQAHPPYS GHHPVPHEAR YRDKRVHDYD MRVDDFLRRT QAVVSGRRSR PRERDRERER DRPRDNRRDR ERDRGRDRER ERERICDRDR DRGERGRYRR. It is sometimes possible for the material contained within the vial of "Ythdc1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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