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WB (Western Blot)
(Detection of human ZEB2/SIP by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB2 recombinant monoclonal antibody (AAA213586 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 .)
ZEB2/SIP recombinant antibody
Rabbit anti-ZEB2/SIP Recombinant Monoclonal Antibody [BLR115H]
Gene Names
ZEB2; SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
ZEB2/SIP, Recombinant Antibody; Rabbit anti-ZEB2/SIP Recombinant Monoclonal Antibody [BLR115H]; ZFHX1B; Zinc finger homeobox protein 1b; zinc finger homeobox 1b; SMADIP1; zinc finger E-box-binding homeobox 2; SMAD interacting protein 1; SIP-1; SIP1; HSPC082; Smad-interacting protein 1; Zinc finger E-box-binding homeobox 2; zinc finger E-box binding homeobox 2; ZEB2/SIP recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR115H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for ZEB2/SIP recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1164 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human ZEB2/SIP by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB2 recombinant monoclonal antibody (AAA213586 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 .)
WB (Western Blot)
(Detection of human ZEB2/SIP by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, MDA-MB-435, HeLa, MOLT-4, HEK293T, RKO, UACC-257, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB2 recombinant monoclonal antibody (AAA213586 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 .)
IP (Immunoprecipitation)
(Detection of human ZEB2/SIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ZEB2 recombinant monoclonal antibody (AAA213586 lot 1) used for IP at 20 ul/mg lysate. ZEB2 was also immunoprecipitated by rabbit anti-ZEB2 antibodies and For blotting immunoprecipitated ZEB2, AAA213586 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human ZEB2/SIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-ZEB2 recombinant monoclonal antibody (AAA213586 lot 1) used for IP at 20 ul/mg lysate. ZEB2 was also immunoprecipitated by rabbit anti-ZEB2 antibodies and For blotting immunoprecipitated ZEB2, AAA213586 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohiostchemistry)
(Detection of human ZEB2/SIP in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody (AAA213586 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human ZEB2/SIP in FFPE prostate carcinoma by immunohistochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody (AAA213586 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ZEB2/SIP in FFPE MDA-MB-435 cells by immunocytochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody (AAA213586 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human ZEB2/SIP in FFPE MDA-MB-435 cells by immunocytochemistry. Antibody: Rabbit anti-ZEB2/SIP recombinant monoclonal antibody (AAA213586 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for ZEB2/SIP recombinant antibody
Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].
Product Categories/Family for ZEB2/SIP recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,806 Da
NCBI Official Full Name
zinc finger E-box-binding homeobox 2 isoform 1
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 2
NCBI Official Symbol
ZEB2
NCBI Official Synonym Symbols
SIP1; SIP-1; ZFHX1B; HSPC082; SMADIP1
NCBI Protein Information
zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; SMAD interacting protein 1; Smad-interacting protein 1
UniProt Protein Name
Zinc finger E-box-binding homeobox 2
UniProt Gene Name
ZEB2
UniProt Synonym Gene Names
KIAA0569; SIP1; ZFHX1B; ZFX1B; SMADIP1
UniProt Entry Name
ZEB2_HUMAN
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Product Notes
The ZEB2/SIP zeb2 (Catalog #AAA213586) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-ZEB2/SIP Recombinant Monoclonal Antibody [BLR115H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZEB2/SIP can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the ZEB2/SIP zeb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZEB2/SIP, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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