Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ZFYVE28 Mouse Monoclonal Antibody (LST2/2426) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Mouse anti-Human ZFYVE28 (Zinc Finger FYVE-type containing 28) Monoclonal Antibody | anti-ZFYVE28 antibody
ZFYVE28 (Zinc Finger FYVE-type containing 28)
Gene Names
ZFYVE28; LST2; LYST2
Reactivity
Human
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
ZFYVE28 (Zinc Finger FYVE-type containing 28), Antibody; ZFYVE28 (Zinc Finger FYVE-type containing 28); Lateral signaling target protein 2 homolog; LST2; LYST2; Zinc finger FYVE domain-containing protein 28 (ZFYVE28); anti-ZFYVE28 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
LST2/2426
Specificity
ZFYVE28 belongs to the lst-2 family. It contains 1 FYVE-type zinc finger. The FYVE-type zinc finger mediates the interaction with phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes when not mono-ubiquitinated at Lys-87. Mono-ubiquitination at Lys-87 prevents binding to phosphatidylinositol 3-phosphate (PI3P) and localization to early endosome membranes. ZFYVE28 is a negative regulator of epidermal growth factor receptor (EGFR) signaling. It acts by promoting EGFR degradation in endosomes when not mono-ubiquitinated. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
538
Applicable Applications for anti-ZFYVE28 antibody
IHC (Immunohistochemistry)
Positive Control
PC3 cells. Testis, Brain, Colon, Kidney or Stomach.
Immunogen
Recombinant full-length human ZFYVE28 protein
Cellular Localization
Cytoplasmic
Hu-chromosome Location
4p16.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ZFYVE28 Mouse Monoclonal Antibody (LST2/2426) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ZFYVE28 Mouse Monoclonal Antibody (LST2/2426) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified ZFYVE28 Mouse Monoclonal Antibody (LST2/2426). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified ZFYVE28 Mouse Monoclonal Antibody (LST2/2426). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ZFYVE28 Mouse Monoclonal Antibody (LST2/2426))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ZFYVE28 Mouse Monoclonal Antibody (LST2/2426))
References
Monoubiquitinylation regulates endosomal localization of Lst2, a negative regulator of EGF receptor signaling.Dev. Cell 16:687-698, 2009.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96kDa
NCBI Official Full Name
zinc finger FYVE-type containing 28 L homeolog
NCBI Official Synonym Full Names
zinc finger FYVE-type containing 28
NCBI Official Symbol
ZFYVE28
NCBI Official Synonym Symbols
LST2; LYST2
NCBI Protein Information
lateral signaling target protein 2 homolog
Similar Products
Product Notes
The ZFYVE28 (Catalog #AAA215170) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZFYVE28 (Zinc Finger FYVE-type containing 28) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZFYVE28 (Zinc Finger FYVE-type containing 28) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ZFYVE28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZFYVE28 (Zinc Finger FYVE-type containing 28), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.