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ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZW10 mAb. [Lot No. 3363C4a-1])
Mouse anti-Human ZW10 Monoclonal Antibody | anti-ZW10 antibody
Mouse monoclonal antibody Anti-Human ZW10
Gene Names
ZW10; HZW10; KNTC1AP
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Dot Blot
Synonyms
ZW10, Antibody; Mouse monoclonal antibody Anti-Human ZW10; Homo sapiens ZW10, kinetochore associated, homolog (Drosophila) (ZW10); HZW10; KNTC1AP; MGC149821; anti-ZW10 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3363C4a
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
2964
Applicable Applications for anti-ZW10 antibody
WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), DB (Dot Blot)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZW10 mAb. [Lot No. 3363C4a-1])
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-ZW10 mAb. [Lot No. 3363C4a-1])
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ZW10 mAb. [Lot No. 3363C4a-1]Predicted molecular weight: 88 kDa)
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ZW10 mAb. [Lot No. 3363C4a-1]Predicted molecular weight: 88 kDa)
WB (Western Blot)
(Detection of ZW10 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3363C4a-1]Predicted molecular weight: 88 kDa)
WB (Western Blot)
(Detection of ZW10 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 3363C4a-1]Predicted molecular weight: 88 kDa)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ZW10 monoclonal antibody.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ZW10 monoclonal antibody.)
Related Product Information for anti-ZW10 antibody
The interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway. [UniProtKB Function]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76,807 Da
NCBI Official Full Name
Homo sapiens zw10 kinetochore protein (ZW10), mRNA
NCBI Official Synonym Full Names
zw10 kinetochore protein
NCBI Official Symbol
ZW10
NCBI Official Synonym Symbols
HZW10; KNTC1AP
NCBI Protein Information
centromere/kinetochore protein zw10 homolog; ZW10 homolog, centromere/kinetochore protein; ZW10, kinetochore associated, homolog; zeste white 10 homolog
UniProt Protein Name
Centromere/kinetochore protein zw10 homolog
UniProt Gene Name
ZW10
UniProt Entry Name
ZW10_HUMAN
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Product Notes
The ZW10 zw10 (Catalog #AAA36435) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human ZW10 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZW10 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), ICC (Immunocytochemistry), DB (Dot Blot). Researchers should empirically determine the suitability of the ZW10 zw10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZW10, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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