Mouse CD4 Antibody | anti-CD4 antibody

Anti-CD4 [CE9.1 (Clenoliximab)], Mouse IgG1

Cat. Num. AAA72237

• Gene Names: CD4; CD4mut
• Reactivity: Human, chimpanzee
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
• Purity: Protein A affinity purified
• Synonyms: CD4; N/A; Anti-CD4 [CE9.1 (Clenoliximab)], Mouse IgG1; Anti-CD4 [CE9.1 (Clenoliximab)]; p55; T cell surface antign T4/Leu-3; anti-CD4 antibody

• Host: Mouse
• Reactivity: Human, chimpanzee
• Isotype: Mouse IgG1, Lambda
• Clone Number: CE9.1 (Clenoliximab)
• Specificity: The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD4 antibody: IHC (Immunohistochemistry), Blocking, FCM/FACS (Flow Cytometry), ELISA
• Immunogen: Domain 1 of human CD4.
• Origin Pub PMID: 1369023
• Chimeric Use Statement: This is a research-grade biosimilar. This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 (primatized) format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (CE9.1, AAA72237) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Clenoliximab (AAA72237-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

IHC (Immunohiostchemistry)

(Immunohistochemical staining of guinea pig skin using anti-CD4 antibody (AAA72237) CE9.1 (Clenoliximab) Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (AAA72237-13.0) was used to stain samples at a concentration of 3 ug/ml.)

WB (Western Blot)

(Western Blot using anti-CD4 antibody CE9.1 (Clenoliximab) (AAA72237) Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (AAA72237-13.0) at 1.5 ug/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. AAA72237-13.0 successfully detected CD4 in human thyroid tissue samples.)

Related Product Information for anti-CD4 antibody

The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as "primatized", as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and gamma4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues. The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec.

Product Categories/Family for anti-CD4 antibody

immunology; immunosuppression; rheumatology; biosimilar

References

Newman et al. Primatization of recombinant antibodies for immunotherapy of human diseases: a macaque/human chimeric antibody against human CD4. Biotechnology (NY). 1992 Nov;10(11):1455-60. PMID:1369023

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN