Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of GnRH. No significant cross-reactivity or interference between GnRH and analogues was observed.
Sequence Length
366
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
5.11pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GnRH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GnRH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Related Product Information for INHA clia kit
Intended Uses: The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of GnRH in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with An antibody specific to GnRH. A competitive inhibition reaction is launched between biotin labeled GnRH and unlabeled GnRH (Standards or samples) with the pre-coated antibody specific to GnRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GnRH in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the GnRH level in the sample or standard.
Principle of the Assay: The microplate provided in this kit has been pre-coated with An antibody specific to GnRH. A competitive inhibition reaction is launched between biotin labeled GnRH and unlabeled GnRH (Standards or samples) with the pre-coated antibody specific to GnRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GnRH in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the GnRH level in the sample or standard.
Product Categories/Family for INHA clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,550 Da
NCBI Official Full Name
inhibin alpha chain isoform 1 preproprotein
NCBI Official Synonym Full Names
inhibin alpha
NCBI Official Symbol
Inha
NCBI Official Synonym Symbols
AW555078
NCBI Protein Information
inhibin alpha chain
UniProt Protein Name
Inhibin alpha chain
UniProt Gene Name
Inha
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Product Notes
The Mouse INHA inha (Catalog #AAA149035) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA149035 CLIA Kit recognizes Mouse INHA. It is sometimes possible for the material contained within the vial of "Inhibin A (INHA), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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