Mouse Matrix Metalloproteinase 13 (MMP13) CLIA Kit | MMP13 clia kit

Mouse Matrix Metalloproteinase 13 (MMP13) CLIA Kit

Cat. Num. AAA188848

• Gene Names: Mmp13; Clg; Mmp1; MMP-13
• Reactivity: Mouse
• Applications: CLIA (Chemiluminescence Immunoassay)
• Synonyms: Matrix Metalloproteinase 13 (MMP13); N/A; Mouse Matrix Metalloproteinase 13 (MMP13) CLIA Kit; Matrix Metalloproteinase 13; CLG3; Collagenase 3; MMP13 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 13 (MMP13).; No significant cross-reactivity or interference between Matrix Metalloproteinase 13 (MMP13) and analogues was observed.
• Sequence Length: 472
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.1pg/mL

• Applicable Applications for MMP13 clia kit: CLIA (Chemiluminescence Immunoassay)
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 13 (MMP13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 13 (MMP13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 13 (MMP13) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

NCBI and Uniprot Product Information

• NCBI GI #: 6678896
• NCBI GeneID: 17386
• NCBI Accession #: NP_032633.1
• NCBI GenBank Nucleotide #: NP_032633.1
• UniProt Accession #: P33435
• NCBI Official Full Name: collagenase 3 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 13
• NCBI Official Symbol: Mmp13
• NCBI Official Synonym Symbols: Clg; Mmp1; MMP-13
• NCBI Protein Information: collagenase 3
• UniProt Protein Name: Collagenase 3
• UniProt Gene Name: Mmp13
• UniProt Synonym Gene Names: MMP-13