Standard Curve (Sample)
Mouse Pigment Epithelium Derived Factor (PEDF) CLIA Kit | PEDF clia kit
Mouse Pigment Epithelium Derived Factor (PEDF) CLIA Kit
Gene Names
SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
Reactivity
Mouse
Synonyms
Pigment Epithelium Derived Factor (PEDF); N/A; Mouse Pigment Epithelium Derived Factor (PEDF) CLIA Kit; SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3; PEDF clia kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF).
No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.
No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.
Sequence Length
418
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.14-100ng/mL
Sensitivity
0.05ng/mL
Application
Chemiluminescent immunoassay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Pigment Epithelium Derived Factor (PEDF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PEDF clia kit
The microplate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pigment Epithelium Derived Factor (PEDF) level in the sample or standard.;
Product Categories/Family for PEDF clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,312 Da
NCBI Official Full Name
pigment epithelium-derived factor
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
NCBI Official Symbol
SERPINF1
NCBI Official Synonym Symbols
OI6; OI12; PEDF; EPC-1; PIG35
NCBI Protein Information
pigment epithelium-derived factor
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Gene Name
SERPINF1
UniProt Synonym Gene Names
PEDF; PEDF
UniProt Entry Name
PEDF_HUMAN
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Product Notes
The Mouse PEDF serpinf1 (Catalog #AAA130279) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA130279 CLIA Kit recognizes Mouse PEDF. It is sometimes possible for the material contained within the vial of "Pigment Epithelium Derived Factor (PEDF), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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