Standard Curve (Sample)
(Typical Standard Curve for STC1 CLIA (Sandwich))
Mouse Stanniocalcin 1 (STC1) CLIA Kit | STC1 clia kit
Mouse Stanniocalcin 1 (STC1) CLIA Kit
Gene Names
Stc1; Stc
Reactivity
Mouse
Synonyms
Stanniocalcin 1 (STC1); N/A; Mouse Stanniocalcin 1 (STC1) CLIA Kit; STC1 CLIA kit (Mouse); STC1 clia kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Stanniocalcin 1 (STC1). No significant cross-reactivity or interference between Stanniocalcin 1 (STC1) and analogues was observed.
Reactive with Mouse STC1/Stanniocalcin 1
Reactive with Mouse STC1/Stanniocalcin 1
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Method
Chemiluminescence
Featured Series
SE kit
Detection Range
13.7-10,000pg/mL
Sensitivity
4.9pg/mL
Sample Size
100uL
Assay Time
2h, 40min
Function
Detects protein (regular version)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stanniocalcin 1 (STC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stanniocalcin 1 (STC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stanniocalcin 1 (STC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C
4. Aspirate and wash 3 times
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
6. Aspirate and wash 5 times
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37 degree C
8. Read RLU value immediately.
Test Principle
The microplate provided in this kit has been pre-coated with an antibody specific to Stanniocalcin 1 (STC1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stanniocalcin 1 (STC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stanniocalcin 1 (STC1) level in the sample or standard.
Research Area
Endocrinology
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
(Typical Standard Curve for STC1 CLIA (Sandwich))
Standard Curve (Sample)
(Typical Standard Curve for STC1 CLIA (Sandwich))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,480 Da
NCBI Official Full Name
stanniocalcin-1
NCBI Official Synonym Full Names
stanniocalcin 1
NCBI Official Symbol
Stc1
NCBI Official Synonym Symbols
Stc
NCBI Protein Information
stanniocalcin-1
UniProt Protein Name
Stanniocalcin-1
UniProt Gene Name
Stc1
UniProt Synonym Gene Names
Stc; STC-1
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Product Notes
The Mouse STC1 stc1 (Catalog #AAA161504) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161504 CLIA Kit recognizes Mouse STC1. It is sometimes possible for the material contained within the vial of "Stanniocalcin 1 (STC1), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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