Mouse IL-2R Antibody | anti-IL-2R antibody

Anti-IL-2R [Daclizumab], Mouse IgG1

Cat. Num. AAA72231

• Gene Names: IL2RA; CD25; IL2R; TCGFR; IDDM10
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A affinity purified
• Synonyms: IL-2R; N/A; Anti-IL-2R [Daclizumab], Mouse IgG1; Anti-IL-2R [Daclizumab]; CD25; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL-2-RA; IL2-RA; TAC antigen; p55; anti-IL-2R antibody

• Host: Mouse
• Reactivity: Human, Rhesus Monkey, Cynomolgus Monkey
• Isotype: Mouse IgG1, Kappa
• Clone Number: Daclizumab
• Specificity: Binds to p55 chain of IL2R on human T cells, Tac antigen, which is expressed on T cells activated by mitogens, soluble antigens, and alloantigens but not on resting T cells, thymocytes, B cells, monocytes, activated B cells, leukemic T cell blasts from patients with acute lymphoblastic leukemia, and long-term T cell lines.
• Purity/Purification: Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IL-2R antibody: Blocking, FCM/FACS (Flow Cytometry)
• Immunogen: Cultured T-cells derived from a patient with Mycosis fungoides.
• Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

FCM/FACS (Flow Cytometry)

(Flow-cytometry using the anti-CD25 (IL2R) research biosimilar antibody Daclizumab (AAA72231) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab (AAA72231-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)

WB (Western Blot)

(Western Blot using anti-IL2R antibody AAA72231 Mouse lipocyte (lane 1), mouse spleen (lane 2), mouse thymus (lane 3), mouse lymph node (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with AAA72231-10.0 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (31 kDa) is seen in the human-derived sample, as well as further bands that are specific to human.)

IHC (Immunohistochemisry)

(Immunohistochemical staining of rat spleen using anti-IL2R antibody AAA72231 Formalin fixed rat spleen slices were were stained with AAA72231-10.0 at 5 ug/ml.)

FCM/FACS (Flow Cytometry)

(Flow-cytometry using anti-IL-2R antibody Daclizumab (AAA72231) Rhesus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab (AAA72231-23.0, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.)

FCM/FACS (Flow Cytometry)

(Flow-cytometry using anti-IL-2R antibody Daclizumab (AAA72231) Cynomolgus monkey lymphocytes were stained with an isotype control or the rabbit-chimeric version of Daclizumab (AAA72231-23.0, blue) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FlowJo single-cell flow cytometer.)

Product Categories/Family for anti-IL-2R antibody

biosimilar

References

Queen C, Schneider WP, Selick HE, Payne PW, Landolfi NF, Duncan JF, Avdalovic NM, Levitt M, Junghans R, Waldmann TA. A humanized antibody that binds to the interleukin 2 receptor. Proc Natl Acad Sci U S A. 1989 Dec;86(24):10029-33. PMID:2513570

NCBI and Uniprot Product Information

• NCBI GI #: 4557667
• NCBI GeneID: 3559
• NCBI Accession #: NP_000408.1
• NCBI GenBank Nucleotide #: NM_000417.2
• UniProt Accession #: P01589; Q5W007
• Molecular Weight: 30,819 Da
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha
• NCBI Official Symbol: IL2RA
• NCBI Official Synonym Symbols: CD25; IL2R; TCGFR; IDDM10
• NCBI Protein Information: interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• UniProt Gene Name: IL2RA
• UniProt Synonym Gene Names: IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_HUMAN