Standard Curve (Sample)
Mouse Acetylcholine receptor subunit alpha (CHRNA1) ELISA Kit | CHRNA1 elisa kit
Mouse Acetylcholine receptor subunit alpha (CHRNA1) ELISA Kit
Gene Names
CHRNA1; ACHRA; ACHRD; CHRNA; CMS2A; FCCMS; SCCMS
Reactivity
Mouse
Synonyms
Acetylcholine receptor subunit alpha (CHRNA1); N/A; Mouse Acetylcholine receptor subunit alpha (CHRNA1) ELISA Kit; CHRNA1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CHRNA1 elisa kit
Intended Uses: This CHRNA1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CHRNA1. This ELISA kit for research use only!
Principle of the Assay: CHRNA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CHRNA1 antibody and an CHRNA1-HRP conjugate. The assay sample and buffer are incubated together with CHRNA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRNA1 concentration since CHRNA1 from samples and CHRNA1-HRP conjugate compete for the anti-CHRNA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRNA1 from the sample, fewer sites are left to bind CHRNA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRNA1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CHRNA1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CHRNA1 antibody and an CHRNA1-HRP conjugate. The assay sample and buffer are incubated together with CHRNA1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHRNA1 concentration since CHRNA1 from samples and CHRNA1-HRP conjugate compete for the anti-CHRNA1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHRNA1 from the sample, fewer sites are left to bind CHRNA1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHRNA1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CHRNA1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,839 Da
NCBI Official Full Name
acetylcholine receptor subunit alpha isoform a
NCBI Official Synonym Full Names
cholinergic receptor, nicotinic, alpha 1 (muscle)
NCBI Official Symbol
CHRNA1
NCBI Official Synonym Symbols
ACHRA; ACHRD; CHRNA; CMS2A; FCCMS; SCCMS
NCBI Protein Information
acetylcholine receptor subunit alpha; nicotinic cholinergic receptor alpha 1; muscle nicotinic acetylcholine receptor; nicotinic acetylcholine receptor alpha subunit; acetylcholine receptor, nicotinic, alpha 1 (muscle); cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)
UniProt Protein Name
Acetylcholine receptor subunit alpha
UniProt Gene Name
CHRNA1
UniProt Synonym Gene Names
ACHRA; CHNRA
UniProt Entry Name
ACHA_HUMAN
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Product Notes
The Mouse CHRNA1 chrna1 (Catalog #AAA245727) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245727 ELISA Kit recognizes Mouse CHRNA1. It is sometimes possible for the material contained within the vial of "Acetylcholine receptor subunit alpha (CHRNA1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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