Standard Curve (Sample)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Mouse Acetylcholinesterase (ACHE) ELISA Kit | ACHE elisa kit
Mouse Acetylcholinesterase (ACHE) ELISA Kit
Reactivity
Mouse
Synonyms
Acetylcholinesterase (ACHE); N/A; Mouse Acetylcholinesterase (ACHE) ELISA Kit; AChE; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; ACHE elisa kit
Reactivity
Mouse
Specificity
Mouse Acetylcholinesterase (ACHE) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse AChE. No significant cross-reactivity or interference between Mouse AChE and analogues was observed.
Sequence Length
614
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse Acetylcholinesterase (ACHE) Standard Curve.)
Related Product Information for ACHE elisa kit
Description: This Mouse Acetylcholinesterase (ACHE) ELISA Kit employs a two-site sandwich ELISA to quantitate AChE in samples. An antibody specific for AChE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AChE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AChE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AChE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Acetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.
Background: Acetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. AChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acetylcholinesterase
NCBI Official Synonym Full Names
acetylcholinesterase
NCBI Official Symbol
Ache
NCBI Protein Information
acetylcholinesterase
UniProt Protein Name
Acetylcholinesterase
UniProt Gene Name
Ache
UniProt Synonym Gene Names
AChE
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Product Notes
The Mouse ACHE ache (Catalog #AAA332395) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332395 ELISA Kit recognizes Mouse ACHE. It is sometimes possible for the material contained within the vial of "Acetylcholinesterase (ACHE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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