Mouse Acyl-CoA dehydrogenase family member 9, mitochondrial (ACAD9) ELISA Kit | ACAD9 elisa kit

Mouse Acyl-CoA dehydrogenase family member 9, mitochondrial (ACAD9) ELISA Kit

Cat. Num. AAA246836

• Gene Names: ACAD9; NPD002
• Reactivity: Mouse
• Synonyms: Acyl-CoA dehydrogenase family member 9, mitochondrial (ACAD9); N/A; Mouse Acyl-CoA dehydrogenase family member 9, mitochondrial (ACAD9) ELISA Kit; ACAD9 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ACAD9. No significant cross-reactivity or interference between ACAD9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACAD9 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ACAD9 elisa kit

Principle of the Assay: ACAD9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACAD9 antibody and an ACAD9-HRP conjugate. The assay sample and buffer are incubated together with ACAD9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACAD9 concentration since ACAD9 from samples and ACAD9-HRP conjugate compete for the anti-ACAD9 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACAD9 from the sample, fewer sites are left to bind ACAD9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACAD9 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ACAD9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ACAD9. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 21361497
• NCBI GeneID: 28976
• NCBI Accession #: NP_054768.2
• NCBI GenBank Nucleotide #: NM_014049.4
• Molecular Weight: 68,760 Da
• NCBI Official Full Name: acyl-CoA dehydrogenase family member 9, mitochondrial
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase family, member 9
• NCBI Official Symbol: ACAD9
• NCBI Official Synonym Symbols: NPD002
• NCBI Protein Information: acyl-CoA dehydrogenase family member 9, mitochondrial; very-long-chain acyl-CoA dehydrogenase VLCAD; acyl-Coenzyme A dehydrogenase family, member 9
• UniProt Protein Name: Acyl-CoA dehydrogenase family member 9, mitochondrial
• UniProt Gene Name: ACAD9
• UniProt Synonym Gene Names: ACAD-9
• UniProt Entry Name: ACAD9_HUMAN