Standard Curve (Sample)
Mouse Adiponectin ELISA Kit | ADIPOQ elisa kit
Adiponectin: Mouse (ELISA)
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Mouse
Synonyms
Adiponectin; N/A; Adiponectin: Mouse (ELISA); Mouse adiponectin; ADIPOQ elisa kit
Reactivity
Mouse
Specificity
The antibodies used in this assay are specific to mouse adiponectin and do not cross-react with human adiponectin, and other cytokine or hormone molecules.
Sequence Length
244
Samples
Serum, plasma and cell culture supernate samples
Assay Type
Quantitative Sandwich
Sensitivity
3.12 ng/ml
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ADIPOQ elisa kit
Intended Uses: This Mouse Adiponectin ELISA kit is designed for quantification of mouse adiponectin in serum, plasma, adipocyte extracts and cell culture media.
Principle of the Assay: This assay is a sandwich ELISA using affinity-purified polyclonal antibodies against mouse adiponectin. The immunoplate is pre-coated with anti-mouse adiponectin capture antibody. Standards and samples are pipetted into the wells and any mouse adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue1. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose2, improve insulin sensitivity3, alleviate fatty liver4 and prevent atherosclerosis5. The protein is posttranslationally modified by hydroxylation and glycosylation6, and forms three different oligomeric complexes in the circulation7. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers1. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases. Low adiponectin concentrations were found to be predictive of a future reduction in insulin sensitivity and cardiovascular disorders. Administration of the anti-diabetic drugs thiazolidinediones (TZDs) raises circulating adiponectin levels8. In addition, low plasma adiponectin levels are also associated with nonalcoholic steatohepatitis (NASH) and certain types of cancers.
Principle of the Assay: This assay is a sandwich ELISA using affinity-purified polyclonal antibodies against mouse adiponectin. The immunoplate is pre-coated with anti-mouse adiponectin capture antibody. Standards and samples are pipetted into the wells and any mouse adiponectin present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-linked polyclonal antibody specific for mouse adiponectin is added to the wells. After a final wash step, an HRP substrate solution is added and colour develops in proportion to the amount of mouse adiponectin bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse adiponectin, the unknown sample concentration can be interpolated from a reference curve included in each assay.
Background/Introduction: Adiponectin, also known as apM1, Acrp30, GBP28 and adipoQ, is a circulating hormone predominantly produced from adipose tissue1. Many pharmacological studies demonstrated that this protein possesses potent anti-diabetic, anti-atherogenic and anti-inflammatory functions. Supplement of adiponectin protein can decrease blood glucose2, improve insulin sensitivity3, alleviate fatty liver4 and prevent atherosclerosis5. The protein is posttranslationally modified by hydroxylation and glycosylation6, and forms three different oligomeric complexes in the circulation7. Many clinical studies demonstrated that plasma adiponectin is an useful biomarker for metabolic syndrome, nonalcoholic steatohepatitis and certain type of cancers1. Decreased circulating levels of plasma adiponectin (hypoadiponectinaemia) are associated with increased body mass index (BMI), decreased insulin sensitivity, less favourable plasma lipid profiles, increased levels of inflammatory markers and increased risk for the development of type 2 diabetes, hypertension, and coronary heart diseases. Low adiponectin concentrations were found to be predictive of a future reduction in insulin sensitivity and cardiovascular disorders. Administration of the anti-diabetic drugs thiazolidinediones (TZDs) raises circulating adiponectin levels8. In addition, low plasma adiponectin levels are also associated with nonalcoholic steatohepatitis (NASH) and certain types of cancers.
References
1. Adiponectin: mechanistic insights and clinical implications. Turer AT & Scherer PE. Diabetologia. 2012 55(9):2319-26.
2. Protective role of adiponectin in cardiovascular disease. Shibata R, Murohara T, Ouchi N. Curr Med Chem. 2012 19(32):5459-66
3. Adiponectin, an anti-carcinogenic hormone A systematic review on breast, colorectal, liver and prostate cancer. Perrier S, Jardé T.Curr Med Chem. 2012 19(32):5501-12
2. Protective role of adiponectin in cardiovascular disease. Shibata R, Murohara T, Ouchi N. Curr Med Chem. 2012 19(32):5459-66
3. Adiponectin, an anti-carcinogenic hormone A systematic review on breast, colorectal, liver and prostate cancer. Perrier S, Jardé T.Curr Med Chem. 2012 19(32):5501-12
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
ADIPOQ
NCBI Official Synonym Symbols
ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
NCBI Protein Information
adiponectin
UniProt Protein Name
Adiponectin
UniProt Gene Name
ADIPOQ
UniProt Synonym Gene Names
ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1
UniProt Entry Name
ADIPO_HUMAN
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Product Notes
The Mouse ADIPOQ adipoq (Catalog #AAA60547) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA60547 ELISA Kit recognizes Mouse ADIPOQ. It is sometimes possible for the material contained within the vial of "Adiponectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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