Standard Curve (Sample)
(Mouse ANGPTL3 PicoKine ELISA Kit standard curve)
Mouse ANGPTL3 ELISA Kit | ANGPTL3 elisa kit
Mouse ANGPTL3 PicoKine ELISA Kit
Gene Names
Angptl3; hypl
Reactivity
Mouse
Synonyms
ANGPTL3; N/A; Mouse ANGPTL3 PicoKine ELISA Kit; Angiopoietin-related protein 3; angiopoietin-like 3; ANG 5; ANG-5; ANG5; Angiopoietin 5; Angiopoietin like 3; Angiopoietin related protein 3; Angiopoietin-5; Angiopoietin-like protein 3; ANGL3_HUMAN; ANGPT5; ANL3; FHBL2; OTTHUMP00000010719; ANGPTL3 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse ANGPTL3
Sequence Length
455
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Mouse ANGPTL3 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Mouse ANGPTL3 PicoKine ELISA Kit standard curve)
Related Product Information for ANGPTL3 elisa kit
Principle of the assay: mouse ANGPTL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ANGPTL3 has been precoated onto 96-well plates. Standards(sf21, S17-T455) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ANGPTL3 amount of sample captured in plate.
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
52,543 Da
NCBI Official Full Name
Angiopoietin-related protein 3
NCBI Official Synonym Full Names
angiopoietin-like 3
NCBI Official Symbol
Angptl3
NCBI Official Synonym Symbols
hypl
NCBI Protein Information
angiopoietin-related protein 3; hypolipidemia; angiopoietin-like protein 3
UniProt Protein Name
Angiopoietin-related protein 3
UniProt Gene Name
Angptl3
UniProt Entry Name
ANGL3_MOUSE
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Product Notes
The Mouse ANGPTL3 angptl3 (Catalog #AAA45789) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45789 ELISA Kit recognizes Mouse ANGPTL3. It is sometimes possible for the material contained within the vial of "ANGPTL3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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