Mouse Apolipoprotein H (Apo-H) ELISA Kit | Apoh elisa kit

Mouse Apolipoprotein H (Apo-H) ELISA Kit

Cat. Num. AAA266534

• Gene Names: Apoh; B2GPI; beta2-GPI; beta-2-GPI
• Reactivity: Mouse
• Synonyms: Apolipoprotein H (Apo-H); N/A; Mouse Apolipoprotein H (Apo-H) ELISA Kit; Apoh elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant Mouse Apoh
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <50 pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays): Three samples of known concentration w ere tested in separate assays to assess inter- assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)

Standard Curve (Sample)

Related Product Information for Apoh elisa kit

Background: Apolipoprotein H (Apo-H), previously known as beta2-glycoprotein I and beta-2 glycoprotein 1, is a 38 kDa multifunctional apolipoprotein that in humans is encoded by the APOH gene. This gene is mapped to II EI; II 71.8 Cm. Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections.

Principle of the Assay: To measure Mouse Apoh, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABCHRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance ofthe yellow product at 450nm is linearly proportional to Mouse Apoh in the sample. Read the absorbance ofthe yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Apoh in the sample.

Intended Uses: This kit is a solid-phase immunoassay specially designed to measure Mouse Apoh with a 96-well strip plate that is pre-coated with antibody specific for Apoh. The detection antibody is a biotinylated antibody specific for Apoh. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit includes Mouse Apoh protein as standards.

NCBI and Uniprot Product Information

• NCBI GI #: 160358825
• NCBI GeneID: 11818
• NCBI Accession #: NP_038503.4
• NCBI GenBank Nucleotide #: NM_013475.4
• UniProt Accession #: Q01339
• Molecular Weight: 38,619 Da
• NCBI Official Full Name: beta-2-glycoprotein 1
• NCBI Official Synonym Full Names: apolipoprotein H
• NCBI Official Symbol: Apoh
• NCBI Official Synonym Symbols: B2GPI; beta2-GPI; beta-2-GPI
• NCBI Protein Information: beta-2-glycoprotein 1; apo-H; beta(2)GPI; APC inhibitor; beta-2-glycoprotein I; activated protein C-binding protein
• UniProt Protein Name: Beta-2-glycoprotein 1
• UniProt Gene Name: Apoh
• UniProt Synonym Gene Names: B2gp1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_MOUSE