Mouse ATP synthase subunit alpha, mitochondrial (ATP5A1) ELISA Kit | ATP5A1 elisa kit

Mouse ATP synthase subunit alpha, mitochondrial (ATP5A1) ELISA Kit

Cat. Num. AAA246691

• Gene Names: ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; HEL-S-123m
• Reactivity: Mouse
• Synonyms: ATP synthase subunit alpha, mitochondrial (ATP5A1); N/A; Mouse ATP synthase subunit alpha, mitochondrial (ATP5A1) ELISA Kit; ATP5A1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP5A1. No significant cross-reactivity or interference between ATP5A1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP5A1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ATP5A1 elisa kit

Principle of the Assay: ATP5A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATP5A1 antibody and an ATP5A1-HRP conjugate. The assay sample and buffer are incubated together with ATP5A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP5A1 concentration since ATP5A1 from samples and ATP5A1-HRP conjugate compete for the anti-ATP5A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP5A1 from the sample, fewer sites are left to bind ATP5A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP5A1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This ATP5A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ATP5A1. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for ATP5A1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 382546007
• NCBI GeneID: 498
• NCBI Accession #: NP_001244263.1
• NCBI GenBank Nucleotide #: NM_001257334.1
• Molecular Weight: 57,547 Da
• NCBI Official Full Name: ATP synthase subunit alpha, mitochondrial isoform b
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• NCBI Official Symbol: ATP5A1
• NCBI Official Synonym Symbols: OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; HEL-S-123m
• NCBI Protein Information: ATP synthase subunit alpha, mitochondrial; ATP sythase (F1-ATPase) alpha subunit; ATP synthase alpha chain, mitochondrial; epididymis secretory sperm binding protein Li 123m; mitochondrial ATP synthetase, oligomycin-resistant; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1, cardiac muscle; ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 2, non-cardiac muscle-like 2
• UniProt Protein Name: ATP synthase subunit alpha, mitochondrial
• UniProt Gene Name: ATP5A1
• UniProt Synonym Gene Names: ATP5A; ATP5AL2; ATPM
• UniProt Entry Name: ATPA_HUMAN