Mouse Beta-Endorphin (bEP) ELISA Kit | bEP elisa kit
Mouse Beta-Endorphin (bEP) ELISA Kit
Reactivity
Mouse
Synonyms
Beta-Endorphin (bEP); N/A; Mouse Beta-Endorphin (bEP) ELISA Kit; b-EP; bEP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of bEP. No significant cross-reactivity or interference between bEP and analogues was observed.
Sequence Length
31
Assay Type
Quantitative Competitive
Samples
Mouse serum, plasma, cerebrospinal fluid and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
< 4.74pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level bEP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level bEP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for bEP elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to bEP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled bEP and unlabeled bEP (Standards or samples) with the pre-coated antibody specific to bEP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of bEP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of bEP in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of bEP in mouse serum, plasma, cerebrospinal fluid and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of bEP in mouse serum, plasma, cerebrospinal fluid and other biological fluids.
References
Therapeutic Effects of Licorice and Dried Ginger Decoction on Activity-Based Anorexia in BALB/c AnNCrl Mice; Front Pharmacol; 2020 Dec 8;11:594706.; PMID: 33519458; Do-Hyun Kim; Department of Oriental Medicine, College of Oriental Medicine, Dongshin University, Naju, South Korea.
