Mouse BMP-4 PicoKine ELISA Kit | Bmp4 elisa kit

Mouse BMP-4 PicoKine ELISA Kit

Cat. Num. AAA46175

• Gene Names: Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• Reactivity: Mouse
• Synonyms: BMP-4 PicoKine; N/A; Mouse BMP-4 PicoKine ELISA Kit; Bone morphogenetic protein 4; BMP 2B; BMP 4; BMP2B; BMP2B1; BOMPR4A; Bone morphogenetic protein 2B; DVR4; OTTMUSP00000021065; OTTMUSP00000021066; ZYME; bone morphogenetic protein 4; Bmp4 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse BMP-4
• Sequence Length: 408
• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse BMP-4 in bone tissue, cell culture supernates and serum.

• Immunogen: CHO, S293-R408
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BMP-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. It is recommended that each mouse BMP-4 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|160|556|1526|178|603|1789; Standard deviation|5.12|22.80|85.46|9.43|41.00|132.39; CV(%)|3.2|4.1|5.6|5.3|6.8|7.4
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.045|0.06|0.094|0.165|0.333|0.706|1.437|2.283
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Mouse BMP-4 PicoKine ELISA Kit standard curve)

Related Product Information for Bmp4 elisa kit

Principle of the assay: Mouse BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for BMP-4 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse BMP-4 amount of sample captured in plate.

Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.

NCBI and Uniprot Product Information

• NCBI GI #: 939320322
• NCBI GeneID: 12159
• NCBI Accession #: NP_001303289.1
• NCBI GenBank Nucleotide #: NM_001316360.1
• UniProt Accession #: P21275
• Molecular Weight: 46,497 Da
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_MOUSE