Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit | BRCA2 elisa kit

Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

Cat. Num. AAA137147

• Gene Names: BRCA2; FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
• Reactivity: Mouse
• Synonyms: Breast Cancer Susceptibility Protein 2 (BRCA2); N/A; Mouse Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit; BRCA2; BRCC2; FACD; FAD; FAD1; FANCD; FANCD1; Breast Cancer 2,Early Onset; Breast Cancer Type 2 Susceptibility Protein; Fanconi anemia group D1 protein; BRCA2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Susceptibility Protein 2 (BRCA2).; No significant cross-reactivity or interference between Breast Cancer Susceptibility Protein 2 (BRCA2) and analogues was observed.
• Sequence Length: 3418
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Susceptibility Protein 2 (BRCA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for BRCA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Susceptibility Protein 2 (BRCA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Susceptibility Protein 2 (BRCA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Susceptibility Protein 2 (BRCA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BRCA2 elisa kit

Tumor immunity; Genetic science

NCBI and Uniprot Product Information

• NCBI GI #: 119395734
• NCBI GeneID: 675
• NCBI Accession #: NP_000050.2
• NCBI GenBank Nucleotide #: NM_000059.3
• UniProt Accession #: P51587; O00183; O15008; Q13879; Q5TBJ7
• Molecular Weight: 384,225 Da
• NCBI Official Full Name: breast cancer type 2 susceptibility protein
• NCBI Official Synonym Full Names: breast cancer 2, early onset
• NCBI Official Symbol: BRCA2
• NCBI Official Synonym Symbols: FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2
• NCBI Protein Information: breast cancer type 2 susceptibility protein
• UniProt Protein Name: Breast cancer type 2 susceptibility protein
• UniProt Gene Name: BRCA2
• UniProt Synonym Gene Names: FACD; FANCD1
• UniProt Entry Name: BRCA2_HUMAN