Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit | CALCB elisa kit

Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit

Cat. Num. AAA27301

• Gene Names: CALCB; CALC2; CGRP2; CGRP-II
• Reactivity: Mouse
• Synonyms: Calcitonin gene-related peptide 2 (CALCB); Mouse Calcitonin gene-related peptide 2 (CALCB) ELISA Kit; CALCB elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CALCB. No significant cross-reactivity or interference between CALCB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALCB and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CALCB elisa kit

Principle of the Assay: CALCB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALCB antibody and an CALCB-HRP conjugate. The assay sample and buffer are incubated together with CALCB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCB concentration since CALCB from samples and CALCB-HRP conjugate compete for the anti-CALCB antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCB from the sample, fewer sites are left to bind CALCB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCB concentration in each sample is interpolated from this standard curve.

Intended Uses: This CALCB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CALCB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for CALCB elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 9945304
• NCBI GeneID: 797
• NCBI Accession #: NP_000719.1
• NCBI GenBank Nucleotide #: NM_000728.3
• Molecular Weight: 13,706 Da
• NCBI Official Full Name: calcitonin gene-related peptide 2
• NCBI Official Synonym Full Names: calcitonin-related polypeptide beta
• NCBI Official Symbol: CALCB
• NCBI Official Synonym Symbols: CALC2; CGRP2; CGRP-II
• NCBI Protein Information: calcitonin gene-related peptide 2; beta-CGRP; calcitonin 2; beta-type CGRP; calcitonin gene-related peptide II
• UniProt Protein Name: Calcitonin gene-related peptide 2
• UniProt Gene Name: CALCB
• UniProt Synonym Gene Names: CALC2; Beta-CGRP; CGRP-II
• UniProt Entry Name: CALCB_HUMAN