Mouse CCL21/6Ckine PicoKine ELISA Kit | Gm1987 elisa kit

Mouse CCL21/6Ckine PicoKine ELISA Kit

Cat. Num. AAA46184

• Gene Names: Gm1987; 100038965
• Reactivity: Mouse
• Synonyms: CCL21/6Ckine PicoKine; N/A; Mouse CCL21/6Ckine PicoKine ELISA Kit; C-C motif chemokine 21; 6Ckine; Beta chemokine exodus 2; Chemokine (C-C motif) ligand 21; CKb9; ECL; Efficient Chemoattractant for Lymphocytes; Exodus2; MGC34555; OTTHUMP00000000526; OTTHUMP00000000527; OTTHUMP00000021303; SCYA 21; SCYA21; Secondary lymphoid tissue chemokine; SLC; Small inducible cytokine A21; Small inducible cytokine subfamily A (Cys-Cys), member 21; TCA 4; TCA4; chemokine (C-C motif) ligand 21; Gm1987 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL21
• Sequence Length: 133
• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse CCL21 in cell culture supernates, serum and plasma(heparin, EDTA).

• Immunogen: E Coli, S24-G133
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse CCL21 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL21 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|125|357|556|130|349|618; Standard deviation|6.5|18.56|31.13|8.32|23.03|36.46; CV(%)|5.2|5.9|5.6|6.4|6.6|5.9
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.042|0.115|0.186|0.336|0.598|1.116|1.898|2.604
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Mouse CCL21/6Ckine PicoKine ELISA Kit standard curve)

Related Product Information for Gm1987 elisa kit

Principle of the assay: Mouse CCL21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL21 has been precoated onto 96-well plates. Standards (E Coli, S24-G133) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL21 amount of sample captured in plate.

Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 302488378
• NCBI GeneID: 100504362
• NCBI Accession #: NP_001180596.1
• NCBI GenBank Nucleotide #: NM_001193667.1
• UniProt Accession #: P84444; O09002; O09006; Q3U1J2; Q91V84
• Molecular Weight: 14,558 Da
• NCBI Official Full Name: predicted gene 1987
• NCBI Official Synonym Full Names: predicted gene 1987
• NCBI Official Symbol: Gm1987
• NCBI Official Synonym Symbols: 100038965
• NCBI Protein Information: predicted gene 1987
• UniProt Protein Name: C-C motif chemokine 21a
• UniProt Gene Name: Ccl21a
• UniProt Synonym Gene Names: Scya21; Scya21a; TCA4
• UniProt Entry Name: CC21A_MOUSE