Mouse CCL6/C10 ELISA Kit | Ccl6 elisa kit

Mouse CCL6/C10 PicoKine ELISA Kit

Cat. Num. AAA45831

• Gene Names: Ccl6; c10; MRP-1; Scya6
• Reactivity: Mouse
• Synonyms: CCL6/C10; N/A; Mouse CCL6/C10 PicoKine ELISA Kit; C-C motif chemokine 6; chemokine (C-C motif) ligand 6 ; C10; CCL 6; Chemokine C C motif Ligand 6; MRP1; SCYA6; Small Inducible Cytokine A6; Ccl6 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL6
• Sequence Length: 116
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Mouse CCL6/C10 PicoKine ELISA Kit standard curve)

Related Product Information for Ccl6 elisa kit

Principle of the assay: mouse CCL6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL6 has been precoated onto 96-well plates. Standards(E.coli, G22-A116) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL6 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 6 (CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents.In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

NCBI and Uniprot Product Information

• NCBI GI #: 115197
• NCBI GeneID: 20305
• UniProt Accession #: P27784; Q3U3W3; Q5QNW1; Q99M24; Q9D830
• Molecular Weight: 12,984 Da
• NCBI Official Full Name: C-C motif chemokine 6
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 6
• NCBI Official Symbol: Ccl6
• NCBI Official Synonym Symbols: c10; MRP-1; Scya6
• NCBI Protein Information: C-C motif chemokine 6; CC chemokine C10; small inducible cytokine A6; small-inducible cytokine A6
• UniProt Protein Name: C-C motif chemokine 6
• UniProt Gene Name: Ccl6
• UniProt Synonym Gene Names: C10; Scya6
• UniProt Entry Name: CCL6_MOUSE