Mouse CD274 molecule ELISA Kit | CD274 elisa kit
Mouse Programmed cell death 1 ligand 1, CD274 ELISA Kit
Gene Names
Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
Reactivity
Mouse
Synonyms
CD274 molecule; N/A; Mouse Programmed cell death 1 ligand 1, CD274 ELISA Kit; Mouse Programmed cell death 1 ligand 1 (CD274) ELISA kit; B7-H; B7H1; MGC142294; MGC142296; PD-L1; PDCD1L1; PDCD1LG1; PDL1; CD274 antigen; programmed cell death 1 ligand 1; CD274 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CD274. No significant cross-reactivity or interference between mouse CD274 and analogues was observed.
Sequence Length
290
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
31.25 pg/ml-2000 pg/ml
Sensitivity
< 7.81 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for CD274 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD274 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD274 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD274 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD274 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,780 Da
NCBI Official Full Name
programmed cell death 1 ligand 1
NCBI Official Synonym Full Names
CD274 antigen
NCBI Official Symbol
Cd274
NCBI Official Synonym Symbols
B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
Cd274
UniProt Synonym Gene Names
B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_MOUSE