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product-image-AAA182449_SC15.jpg Standard Curve (Sample)

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit | CTGF elisa kit

Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit

Gene Names
CTGF; CCN2; NOV2; HCS24; IGFBP8
Reactivity
Mouse
Synonyms
Connective Tissue Growth Factor (CTGF); N/A; Mouse Connective Tissue Growth Factor (CTGF) ELISA Kit; CCN2; HCS24; IGFBP8; NOV2; Insulin-Like Growth Factor-Binding Protein 8; Hypertrophic Chondrocyte-Specific Protein 24; CCN Family Member 2; CTGF elisa kit
Ordering
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of CTGF. No significant cross-reactivity or interference between CTGF and analogues was observed.
Sequence Length
349
Assay Type
Quantitative Sandwich
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
31.2-2,000pg/mL
Sensitivity
< 11.4pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CTGF were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CTGF were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA182449_SC15.jpg Standard Curve (Sample)
Related Product Information for CTGF elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CTGF. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CTGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CTGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CTGF in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,205 Da
NCBI Official Full Name
connective tissue growth factor
NCBI Official Synonym Full Names
connective tissue growth factor
NCBI Official Symbol
CTGF
NCBI Official Synonym Symbols
CCN2; NOV2; HCS24; IGFBP8
NCBI Protein Information
connective tissue growth factor
UniProt Protein Name
Connective tissue growth factor
UniProt Gene Name
CTGF
UniProt Synonym Gene Names
CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

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Product Notes

The Mouse CTGF ctgf (Catalog #AAA182449) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA182449 ELISA Kit recognizes Mouse CTGF. It is sometimes possible for the material contained within the vial of "Connective Tissue Growth Factor (CTGF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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