Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit | CRH elisa kit

Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit

Cat. Num. AAA68114

• Gene Names: Crh; CRF; Gm1347
• Reactivity: Mouse
• Synonyms: Corticotropin Releasing Hormone (CRH); N/A; Mouse Corticotropin Releasing Hormone (CRH) ELISA Kit; CRH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRH. No significant cross-reactivity or interference between CRH and analogues was observed.
• Sequence Length: 187
• Assay Type: Quantitative Competitive
• Samples: Mouse serum, plasma or other biological fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 4.45pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Related Product Information for CRH elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to CRH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled CRH and unlabeled CRH (Standards or samples) with the pre-coated antibody specific to CRH. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of CRH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of CRH in the sample.

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of CRH in mouse serum, plasma or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 45429990
• NCBI GeneID: 12918
• NCBI Accession #: NP_991338.1
• NCBI GenBank Nucleotide #: NM_205769.3
• UniProt Accession #: Q8CIT0; Q14AA2
• Molecular Weight: 20,778 Da
• NCBI Official Full Name: corticoliberin preproprotein
• NCBI Official Synonym Full Names: corticotropin releasing hormone
• NCBI Official Symbol: Crh
• NCBI Official Synonym Symbols: CRF; Gm1347
• NCBI Protein Information: corticoliberin
• UniProt Protein Name: Corticoliberin
• UniProt Gene Name: Crh
• UniProt Synonym Gene Names: Gm1347; CRF