Reactivity
Mouse
Specificity
The Mouse Complement C5 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Complement C5 proteins within the range of 15.6 pg/ml-1000 pg/ml.
Sequence Length
167
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
10 pg/ml.
Preparation and Storage
Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Related Product Information for CXCL15 elisa kit
Principle of the Assay: The Mouse Complement C5 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Complement C5 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Complement C5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Complement C5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Complement C5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Complement C5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,091 Da
NCBI Official Full Name
C-X-C motif chemokine 15
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 15
NCBI Official Symbol
Cxcl15
NCBI Official Synonym Symbols
Il8; weche; Scyb15; lungkine
NCBI Protein Information
C-X-C motif chemokine 15
UniProt Protein Name
C-X-C motif chemokine 15
UniProt Gene Name
Cxcl15
UniProt Synonym Gene Names
Scyb15
