Standard Curve (Sample)
Mouse Cyp1a1 (Cytochrome P450 1A1) ELISA Kit | Cyp1a1 elisa kit
Mouse Cyp1a1 (Cytochrome P450 1A1) ELISA Kit
Gene Names
Cyp1a1; AHH; AHRR; CP11; Cyp1a2; P450-1
Reactivity
Mouse
Synonyms
Cyp1a1 (Cytochrome P450 1A1); N/A; Mouse Cyp1a1 (Cytochrome P450 1A1) ELISA Kit; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; Hydroperoxy icosatetraenoate dehydratase; Cyp1a-1; Cyp1a1; Cyp1a1 elisa kit
Reactivity
Mouse
Specificity
Specifically recognize Cyp1a1, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Cell lysates, tissue homogenates and other biological samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Cyp1a1 elisa kit
Background: Cytochrome P450 (CYP) 1A1 is an extrahepatic monooxygenase involved in the metabolism of endogenous substrates and drugs, as well as the activation of certain toxins and environmental pollutants. CYP1A1 is particularly well known for its ability to biotransform polycyclic aromatic hydrocarbons, such as benzo[a]pyrene in tobacco smoke, into carcinogens.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Cyp1a1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti Cyp1a1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Cyp1a1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Cyp1a1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Cyp1a1 antibody was pre-coated onto the 96-well plate. The biotin conjugated anti Cyp1a1 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Cyp1a1 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Cyp1a1 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,230 Da
NCBI Official Full Name
cytochrome P450 1A1
NCBI Official Synonym Full Names
cytochrome P450, family 1, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp1a1
NCBI Official Synonym Symbols
AHH; AHRR; CP11; Cyp1a2; P450-1
NCBI Protein Information
cytochrome P450 1A1
UniProt Protein Name
Cytochrome P450 1A1
UniProt Gene Name
Cyp1a1
UniProt Synonym Gene Names
Cyp1a-1
UniProt Entry Name
CP1A1_MOUSE
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Product Notes
The Mouse Cyp1a1 cyp1a1 (Catalog #AAA250192) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA250192 ELISA Kit recognizes Mouse Cyp1a1. It is sometimes possible for the material contained within the vial of "Cyp1a1 (Cytochrome P450 1A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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