Mouse Dystrophia Myotonica Protein Kinase (DMPK) ELISA Kit | DMPK elisa kit

Mouse Dystrophia Myotonica Protein Kinase (DMPK) ELISA Kit

Cat. Num. AAA189789

• Gene Names: Dmpk; DM; DMK; Dm15; MDPK; MT-PK
• Reactivity: Mouse
• Synonyms: Dystrophia Myotonica Protein Kinase (DMPK); N/A; Mouse Dystrophia Myotonica Protein Kinase (DMPK) ELISA Kit; DM; DM1; DM1PK; DMK; MDPK; MT-PK; Myotonic Dystrophy Protein Kinase; Myotonin-Protein Kinase; Dystrophia Myotonica 1; Thymopoietin Homolog; DMPK/Dystrophia Myotonica Protein Kinase; DMPK elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DMPK. No significant cross-reactivity or interference between DMPK and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Mouse tissue homogenates, cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DMPK were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DMPK were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life); Shelf Life: 8 months; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for DMPK elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DMPK. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DMPK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DMPK in mouse tissue homogenates, cell lysates and other biological fluids.

Product Categories/Family for DMPK elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 299758507
• NCBI GeneID: 13400
• NCBI Accession #: NP_001177420.1
• NCBI GenBank Nucleotide #: NM_001190491.1
• UniProt Accession #: P54265
• Molecular Weight: 69,602 Da
• NCBI Official Full Name: myotonin-protein kinase isoform 3
• NCBI Official Synonym Full Names: dystrophia myotonica-protein kinase
• NCBI Official Symbol: Dmpk
• NCBI Official Synonym Symbols: DM; DMK; Dm15; MDPK; MT-PK
• NCBI Protein Information: myotonin-protein kinase; DM-kinase; dystrophia myotonica kinase, B15; myotonic dystrophy protein kinase
• UniProt Protein Name: Myotonin-protein kinase
• UniProt Gene Name: Dmpk
• UniProt Synonym Gene Names: Dm15; Mdpk; MT-PK; DMK; MDPK
• UniProt Entry Name: DMPK_MOUSE