Standard Curve (Sample)
(Human EGFR PicoKine ELISA Kit standard curve)
Mouse EGF ELISA Kit | EGF elisa kit
Mouse EGF PicoKine Quick ELISA Kit
Gene Names
Egf; AI790464
Reactivity
Mouse
Applications
ELISA
Synonyms
EGF; N/A; Mouse EGF PicoKine Quick ELISA Kit; EGF elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Egf
Assay Type
Sandwich
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Detection Range
7.8 pg/ml - 500 pg/ml
Sensitivity
<1 pg/ml
Applicable Applications for EGF elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human EGFR PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human EGFR PicoKine ELISA Kit standard curve)
Related Product Information for EGF elisa kit
Background: Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF acts by binding with high affinity to epidermal growth factor receptor(EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.
Principle of the Assay: The Quick Picokine™ Mouse Egf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Mouse Egf protein as standards. To measure Mouse Egf, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Mouse Egf in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Egf in the sample.
Principle of the Assay: The Quick Picokine™ Mouse Egf Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Egf in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Mouse Egf protein as standards. To measure Mouse Egf, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Mouse Egf in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Egf in the sample.
Product Categories/Family for EGF elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
133,072 Da
NCBI Official Full Name
pro-epidermal growth factor isoform 1
NCBI Official Synonym Full Names
epidermal growth factor
NCBI Official Symbol
Egf
NCBI Official Synonym Symbols
AI790464
NCBI Protein Information
pro-epidermal growth factor
UniProt Protein Name
Pro-epidermal growth factor
UniProt Gene Name
Egf
UniProt Synonym Gene Names
EGF
UniProt Entry Name
EGF_MOUSE
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Product Notes
The Mouse EGF egf (Catalog #AAA126783) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126783 ELISA Kit recognizes Mouse EGF. AAA Biotech's EGF can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the EGF egf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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