Mouse EGFR ELISA Kit | EGFR elisa kit

Mouse EGFR PicoKine Quick ELISA Kit

Cat. Num. AAA128095

• Gene Names: Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: EGFR; N/A; Mouse EGFR PicoKine Quick ELISA Kit; Epidermal growth factor receptor; 2.7.10.1; Egfr; EGFR elisa kit

• Reactivity: Mouse
• Samples: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <15pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Immunogen: Expression system for standard: NS0; Immunogen sequence: L25-S647
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for EGFR elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10 ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
  O.D.	0.058	0.104	0.130	0.195	0.355	0.644	1.158	1.908

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/ml)	314	1163	5358	339	1146	5763
  Standard deviation	16.95	60.47	241.11	21.01	75.2%3	305.43
  CV (%)	5.4%	5.2%	4.5%	6.2%	6.6%	5.3%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	314	365	341	349	342	18.45	5.3%
  Sample 2	1163	1083	1036	1179	1115	58.44	5.2%
  Sample 3	5358	5497	5082	4676	5153	313.42	6%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Mouse EGFR PicoKine ELISA Kit standard curve)

Related Product Information for EGFR elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for EGFR elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 46560582
• NCBI GeneID: 13649
• NCBI Accession #: NP_997538.1
• NCBI GenBank Nucleotide #: NM_207655.2
• UniProt Accession #: Q01279
• Molecular Weight: 134,853 Da
• NCBI Official Full Name: epidermal growth factor receptor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: Egfr
• NCBI Official Synonym Symbols: Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: Egfr
• UniProt Entry Name: EGFR_MOUSE