Mouse EGFR ELISA Kit | EGFR elisa kit

Mouse EGFR ELISA Kit

Cat. Num. AAA263114

• Gene Names: Egfr; Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• Reactivity: Mouse
• Synonyms: EGFR; N/A; Mouse EGFR ELISA Kit; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR elisa kit

• Reactivity: Mouse
• Specificity: The Mouse EGFR ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse EGFR proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 1210
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for EGFR elisa kit

Principle of the Assay: The Mouse EGFR ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse EGFR in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse EGFR coated on a 96-well plate. Standards and samples are pipetted into the wells and EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse EGFR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

NCBI and Uniprot Product Information

• NCBI GI #: 46560582
• NCBI GeneID: 13649
• NCBI Accession #: NP_997538.1
• NCBI GenBank Nucleotide #: NM_207655.2
• UniProt Accession #: Q01279
• Molecular Weight: 134,853 Da
• NCBI Official Full Name: epidermal growth factor receptor isoform 1
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: Egfr
• NCBI Official Synonym Symbols: Wa5; wa2; Erbb; Errp; wa-2; Errb1; AI552599; 9030024J15Rik
• NCBI Protein Information: epidermal growth factor receptor
• UniProt Protein Name: Epidermal growth factor receptor
• UniProt Gene Name: Egfr
• UniProt Entry Name: EGFR_MOUSE