Standard Curve (Sample)
Mouse EphA4 ELISA Kit | EphA4 elisa kit
Mouse EphA4 PicoKine ELISA Kit
Gene Names
Epha4; rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
Reactivity
Mouse
Synonyms
EphA4; N/A; Mouse EphA4 PicoKine ELISA Kit; Mouse EphA4; Eph receptor A4; Ephrin type-A receptor 4 (EC:2.7.10.1); Tyrosine-protein kinase receptor MPK-3; Epha4; Tyrosine-protein kinase receptor SEK-1; Sek; Sek1; EphA4 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Epha4
Sequence Length
986
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for EphA4 elisa kit
Principle of the Assay: The Picokine™ Mouse Epha4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Epha4 with a 96-well strip plate that is pre-coated with antibody specific for Epha4. The detection antibody is a biotinylated antibody specific for Epha4. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Epha4 with immunogen: Expression system for standard: NSO, Immunogen sequence: V20-T547. The kit is analytically validated with ready to use reagents. To measure Mouse Epha4, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Epha4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Epha4 in the sample.!!Background/Introduction: EPH receptor A4 (ephrin type-A receptor 4) is a protein that in humans is encoded by the EPHA4 gene. It is mapped to 1 C4; 1 39.55 cM. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ephrin type-A receptor 4
NCBI Official Synonym Full Names
Eph receptor A4
NCBI Official Symbol
Epha4
NCBI Official Synonym Symbols
rb; Sek; Cek8; Hek8; Sek1; Tyro1; AI385584; 2900005C20Rik
NCBI Protein Information
ephrin type-A receptor 4
UniProt Protein Name
Ephrin type-A receptor 4
UniProt Gene Name
Epha4
UniProt Synonym Gene Names
Sek; Sek1
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Product Notes
The Mouse EphA4 epha4 (Catalog #AAA125188) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125188 ELISA Kit recognizes Mouse EphA4. It is sometimes possible for the material contained within the vial of "EphA4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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