Mouse Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit | GPX5 elisa kit

Mouse Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit

Cat. Num. AAA245887

• Gene Names: GPX5; HEL-S-75p
• Reactivity: Mouse
• Synonyms: Epididymal secretory glutathione peroxidase (GPX5); N/A; Mouse Epididymal secretory glutathione peroxidase (GPX5) ELISA Kit; GPX5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GPX5. No significant cross-reactivity or interference between GPX5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPX5 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GPX5 elisa kit

Principle of the Assay: GPX5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GPX5 antibody and an GPX5-HRP conjugate. The assay sample and buffer are incubated together with GPX5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPX5 concentration since GPX5 from samples and GPX5-HRP conjugate compete for the anti-GPX5 antibody binding site. Since the number of sites is limited, as more sites are occupied by GPX5 from the sample, fewer sites are left to bind GPX5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPX5 concentration in each sample is interpolated from this standard curve.

Intended Uses: This GPX5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GPX5. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for GPX5 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4557629
• NCBI GeneID: 2880
• NCBI Accession #: NP_001500.1
• NCBI GenBank Nucleotide #: NM_001509.2
• Molecular Weight: 11,429 Da
• NCBI Official Full Name: epididymal secretory glutathione peroxidase isoform 1
• NCBI Official Synonym Full Names: glutathione peroxidase 5 (epididymal androgen-related protein)
• NCBI Official Symbol: GPX5
• NCBI Official Synonym Symbols: HEL-S-75p
• NCBI Protein Information: epididymal secretory glutathione peroxidase; EGLP; GPx-5; GSHPx-5; epididymal androgen-related protein; epididymis secretory sperm binding protein Li 75p; epididymis-specific glutathione peroxidase-like protein
• UniProt Protein Name: Epididymal secretory glutathione peroxidase
• UniProt Gene Name: GPX5
• UniProt Synonym Gene Names: EGLP; GPx-5; GSHPx-5
• UniProt Entry Name: GPX5_HUMAN