Mouse Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit | FAM3B elisa kit

Mouse Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit

Cat. Num. AAA63865

• Gene Names: Fam3b; 2-21; ORF9; Pander; D16Jhu19e; 9030624C24Rik
• Reactivity: Mouse
• Synonyms: Family With Sequence Similarity 3, Member B (FAM3B); N/A; Mouse Family With Sequence Similarity 3, Member B (FAM3B) ELISA Kit; ORF9; PANDER; PRED44; C21orf11; C21orf76; Cytokine-like protein 2-21; Pancreatic-derived factor; FAM3B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of FAM3B. No significant cross-reactivity or interference between FAM3B and analogues was observed.
• Sequence Length: 235
• Assay Type: Quantitative Sandwich
• Samples: Mouse serum, plasma, tissue homogenates or other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.124ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FAM3B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FAM3B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for FAM3B elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FAM3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FAM3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FAM3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FAM3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FAM3B in mouse serum, plasma, tissue homogenates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 22296880
• NCBI GeneID: 52793
• NCBI Accession #: NP_065647.1
• NCBI GenBank Nucleotide #: NM_020622.3
• UniProt Accession #: Q9D309; O88417
• Molecular Weight: 26,152 Da
• NCBI Official Full Name: protein FAM3B
• NCBI Official Synonym Full Names: family with sequence similarity 3, member B
• NCBI Official Symbol: Fam3b
• NCBI Official Synonym Symbols: 2-21; ORF9; Pander; D16Jhu19e; 9030624C24Rik
• NCBI Protein Information: protein FAM3B
• UniProt Protein Name: Protein FAM3B
• UniProt Gene Name: Fam3b
• UniProt Synonym Gene Names: ORF9; PANDER