Standard Curve (Sample)
Mouse FAS / APO-1 / CD95 ELISA Kit | FAS / APO-1 / CD95 elisa kit
FAS / APO-1 / CD95 Mouse ELISA Kit
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Mouse
Applications
ELISA
Synonyms
FAS / APO-1 / CD95; N/A; FAS / APO-1 / CD95 Mouse ELISA Kit; Mouse FAS / APO-1 / CD95; FAS / APO-1 / CD95 elisa kit
Reactivity
Mouse
Assay Type
Sandwich
Detection Range
31.2 pg/ml-2000 pg/ml
Sensitivity
< 3pg/ml
Applicable Applications for FAS / APO-1 / CD95 elisa kit
ELISA
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FAS / APO-1 / CD95 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-FAS polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FAS polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FAS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FAS can be calculated.
Background: Fas, also known as CD95, APO-1 and TNFRSF6, is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and it has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of Fas with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and it is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.
Background: Fas, also known as CD95, APO-1 and TNFRSF6, is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and it has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of Fas with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and it is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
37,732 Da
NCBI Official Full Name
Fas/Apo-1/CD95
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
FAS
NCBI Official Synonym Symbols
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6; Fas AMA; FAS 827dupA; CD95 antigen; FASLG receptor; apoptosis antigen 1; Delta Fas/APO-1/CD95; APO-1 cell surface antigen; TNF receptor superfamily member 6; apoptosis-mediating surface antigen FAS; Fas (TNF receptor superfamily, member 6); tumor necrosis factor receptor superfamily, member 6
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6
UniProt Gene Name
FAS
UniProt Synonym Gene Names
APT1; FAS1; TNFRSF6
UniProt Entry Name
TNR6_HUMAN
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Product Notes
The Mouse FAS / APO-1 / CD95 fas (Catalog #AAA58936) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA58936 ELISA Kit recognizes Mouse FAS / APO-1 / CD95. AAA Biotech's FAS / APO-1 / CD95 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the FAS / APO-1 / CD95 fas for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FAS / APO-1 / CD95, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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