Standard Curve (Sample)
Mouse FGF1 ELISA Kit | FGF1 elisa kit
Mouse FGF1 ELISA Kit
Gene Names
Fgf1; Fam; Fgfa; Dffrx; Fgf-1
Reactivity
Mouse
Applications
ELISA
Synonyms
FGF1; Mouse FGF1 ELISA Kit; FGFA; Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Endothelial cell growth factor; ECGF; Heparin-binding growth factor 1; HBGF-1; FGF1 elisa kit
Reactivity
Mouse
Specificity
The Mouse FGF1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse FGF1 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
155
Samples
Culture Supernatants, Serum, Plasma
Sensitivity
< 6 pg/ml
Applicable Applications for FGF1 elisa kit
Sandwich ELISA (SE)
Detection Method
Colorimetric
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FGF1 elisa kit
Background: Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
Principle of the Assay: The Mouse FGF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FGF1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse FGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse FGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Mouse FGF1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FGF1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse FGF1 coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse FGF1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,418 Da
NCBI Official Full Name
fibroblast growth factor 1
NCBI Official Synonym Full Names
fibroblast growth factor 1
NCBI Official Symbol
Fgf1
NCBI Official Synonym Symbols
Fam; Fgfa; Dffrx; Fgf-1
NCBI Protein Information
fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic)
UniProt Protein Name
Fibroblast growth factor 1
UniProt Gene Name
Fgf1
UniProt Synonym Gene Names
Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
UniProt Entry Name
FGF1_MOUSE
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Product Notes
The Mouse FGF1 fgf1 (Catalog #AAA17887) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17887 ELISA Kit recognizes Mouse FGF1. AAA Biotech's FGF1 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the FGF1 fgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FGF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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