Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Sequence Length
128
Assay Type
Sandwich
Detection Range
1000 ng/mL-15.6 ng/mL
Sensitivity
Up to 5 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for FDP elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse FDP monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
14,332 Da
NCBI Official Full Name
fibrocyte-derived protein
UniProt Protein Name
Otoraplin
UniProt Gene Name
OTOR
UniProt Synonym Gene Names
FDP; MIAL; UNQ3054/PRO9873
UniProt Entry Name
OTOR_HUMAN