Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit | FGF15 elisa kit
Mouse Fibroblast Growth Factor 15 (FGF15) High Sensitive ELISA Kit
Reactivity
Mouse
Synonyms
Fibroblast Growth Factor 15 (FGF15); N/A; Mouse Fibroblast Growth Factor 15 (FGF15) High Sensitive ELISA Kit; FGF15 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of FGF15.
Assay Type
Quantitative Competitive
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
6.25-100pg/mL
Sensitivity
< 2.62pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF15 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF15 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for FGF15 elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to FGF15 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF15 and unlabeled FGF15 (Standards or samples) with the pre-coated antibody specific to FGF15. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF15 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF15 in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF15 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. FGF15 is the FGF-19 ortholog in mice and they have the same amino acid sequence.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF15 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. FGF15 is the FGF-19 ortholog in mice and they have the same amino acid sequence.
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