Mouse Fibronectin / FN ELISA Kit | FN elisa kit
Fibronectin / FN Mouse ELISA Kit
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
Reactivity
Mouse
Applications
ELISA
Synonyms
Fibronectin / FN; N/A; Fibronectin / FN Mouse ELISA Kit; Mouse Fibronectin / FN; FN elisa kit
Reactivity
Mouse
Assay Type
Sandwich
Samples
Mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants
Detection Range
156 pg/ml-10,000 pg/ml
Sensitivity
< 15 pg/ml
Applicable Applications for FN elisa kit
Quantitative sELISA (EIA)
Application Notes
For quantitative detection of Fibronectin in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Preparation and Storage
Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.
Standard Curve (Sample)
Related Product Information for FN elisa kit
Background: Fibronectin is a high-molecular weight (~440kDa) glycoprotein of the extracellular matrix, belongs to a family of high molecular weight glycoproteins that are present on cell surfaces, in extracellular fluids, connective tissues, and basement membranes. Two major forms of fibronectin exist: a plasma soluble form and a cellular form. It plays a major role in cell adhesion, growth, migration, and differentiation, and it is important for processes such as wound healing and embryonic development. Fibronectin has been implicated in carcinoma development, and also acts as a potential biomarker for radioresistance.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Fibronectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fibronectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fibronectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fibronectin can be calculated.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Fibronectin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fibronectin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fibronectin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fibronectin can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
262,625 Da
NCBI Official Full Name
fibronectin
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2
NCBI Protein Information
fibronectin; cold-insoluble globulin; migration-stimulating factor
UniProt Protein Name
Fibronectin
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN; FN; CIG
UniProt Entry Name
FINC_HUMAN
