Standard Curve (Sample)
Mouse Heparin-binding growth factor 1 (FGF1) ELISA Kit | FGF1 elisa kit
Mouse Heparin-binding growth factor 1 (FGF1) ELISA Kit
Gene Names
FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
Reactivity
Mouse
Synonyms
Heparin-binding growth factor 1 (FGF1); N/A; Mouse Heparin-binding growth factor 1 (FGF1) ELISA Kit; FGF1 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for FGF1 elisa kit
Intended Uses: This HBGF1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HBGF1.
Principle of the Assay: HBGF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBGF1 antibody and an HBGF1-HRP conjugate. The assay sample and buffer are incubated together with HBGF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBGF1 concentration since HBGF1 from samples and HBGF1-HRP conjugate compete for the anti-HBGF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HBGF1 from the sample, fewer sites are left to bind HBGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBGF1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: HBGF1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HBGF1 antibody and an HBGF1-HRP conjugate. The assay sample and buffer are incubated together with HBGF1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HBGF1 concentration since HBGF1 from samples and HBGF1-HRP conjugate compete for the anti-HBGF1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HBGF1 from the sample, fewer sites are left to bind HBGF1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HBGF1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for FGF1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
6,698 Da
NCBI Official Full Name
heparin-binding growth factor 1, partial
NCBI Official Synonym Full Names
fibroblast growth factor 1 (acidic)
NCBI Official Symbol
FGF1
NCBI Official Synonym Symbols
AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha
NCBI Protein Information
fibroblast growth factor 1; heparin-binding growth factor 1; beta-endothelial cell growth factor; endothelial cell growth factor, beta; endothelial cell growth factor, alpha
UniProt Protein Name
Fibroblast growth factor 1
UniProt Gene Name
FGF1
UniProt Synonym Gene Names
FGFA; FGF-1; aFGF; ECGF; HBGF-1
UniProt Entry Name
FGF1_HUMAN
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Product Notes
The Mouse FGF1 fgf1 (Catalog #AAA247037) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA247037 ELISA Kit recognizes Mouse FGF1. It is sometimes possible for the material contained within the vial of "Heparin-binding growth factor 1 (FGF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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