Standard Curve (Sample)
Mouse HRG/HPRG ELISA Kit | HRG/HPRG elisa kit
Mouse HRG/HPRG PicoKine ELISA Kit
Gene Names
HRG; HPRG; HRGP; THPH11
Reactivity
Mouse
Synonyms
HRG/HPRG; N/A; Mouse HRG/HPRG PicoKine ELISA Kit; Mouse HRG; HRG/HPRG elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Hp
Sequence Length
525
Samples
Cell Culture Supernates, Serum And Plasma (Heparin, Edta)
Detection Range
1.56ng/ml-100ng/ml
Sensitivity
<20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HRG/HPRG elisa kit
Principle of the Assay: The Picokine Mouse Hp Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Hp with a 96-well strip plate that is pre-coated with antibody specific for Hp. The detection antibody is a biotinylated antibody specific for Hp. The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Hp with immunogen: Expression system for standard: NSO, Immunogen sequence: V19-N347. The kit is analytically validated with ready to use reagents. To measure Mouse Hp, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Mouse Hp in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Hp in the sample!!Background/Introduction: Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. In clinical settings, the haptoglobulin assay is used to screen for and monitor intravascular hemolytic anemia.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histidine-rich glycoprotein
NCBI Official Synonym Full Names
histidine rich glycoprotein
NCBI Official Symbol
HRG
NCBI Official Synonym Symbols
HPRG; HRGP; THPH11
NCBI Protein Information
histidine-rich glycoprotein
Similar Products
Product Notes
The Mouse HRG/HPRG (Catalog #AAA125028) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125028 ELISA Kit recognizes Mouse HRG/HPRG. It is sometimes possible for the material contained within the vial of "HRG/HPRG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.